When switching from the low-power objective to the high-power objective should you open or close the diaphragm? Explain your answer.

Quesiton : When switching from the low-power objective to the high-power objective should you open or close the diaphragm? Explain your answer.

Answer: eyepiece

When switching from the low-power objective to the high-power objective should you open or close the diaphragm? Explain your answer. Open Diaphragm starting with the most light then lessen the light until the specimen is a clear crisp image. When switching from the low-power objective to the high-power objective should you open or close the di aphragm? Explain your answer. When switching from the low power objective to the high power objective should you open or close the di aphragm? Explain your answer. You should open the diaphragm so you can see clearer and allow more light. The low power objective is used first for two reasons: First focusing the low power objective will insure that the other objectives will be at the proper height. Sun Oct 22 2006 00:00:00 GMT+0530 (IST) · Best Answer : Low power objective is fine tuning and high-power ejective is relatively coarser so I suppose you open up the diaphragm . A high power objective lens is an important part of a microscope that magnifies up to 40 times. This lens is used for observing fine details such as muscle striations and retinal nerve cells. The strength is second only to an oil immersion objective lens which magnifies up to 100 times. Changing from low power to high power increases the magnification of a specimen. The amount an image is magnified is equal to the magnification of the ocular lens or eyepiece multiplied by the magnification of the objective lens. A high power objective also known as a high dry objective is used for observing fine detail. Such details include the striations in skeletal muscle the arrangement of Haversian systems in compact bone and types of nerve cells in the retina. Low power objective lens give you a wider field of view of the particular specimen. It's primary function is to give the viewer a broader range of the image to see other properties of a particular specimen whatever it may be. Next move the high power objective out of position place a small drop of oil on top of the cover slip above the specimen to be viewed and move the oil immersion lens into place. Use the fine adjustment knob to bring the specimen into focus. For a typical compound microscope with a 10X ocular lens and objective lens of 4X 10X 40X and 100X magnification your microscope will have 40X 100X 400X and 1000X magnification depending which objective lens you use.